Praesto SP90 is an agarose-based cation exchange resin, designed for large scale biomolecule purification, including proteins, peptides and oligonucleotides. The relatively large particle size (90 µm) makes Praesto SP90 ideal for capture or intermediate purification of proteins when large sample volumes needs to be processed within a limited time frame.

CONTACT A SPECIALIST

Principal Applications

  • Protein purification
  • MAb Purification

Advantages

  • Excellent dynamic binding capacities for high-productivity and scalability
  • Excellent pressure/flow performance
  • High resolution/selectivity
  • Excellent chemical and physical stability
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Sulfopropyl
Chemical stability All commonly used aqueous buffers. 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid Oxidising agents, cationic detergents
Ionic capacity - medium 0.11 - 0.16 mmol H⁺/ml
Ionic capacity - resin 0.11 - 0.16 mmol/ml
Particle Size - µm 90 µm
Dynamic Binding Capacity (min.) - 6 minutes residence time 50 lgG/ml
Dynamic Binding Capacity (min.)- 3 minutes residence time 50 hlgG/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 800 cm/h
pH stability, CIP (short term) 3 - 14
pH stability, working range 4 - 13
Recommended Storage 4 - 30 °C
Recommended Storage 20% ethanol
Recommended Storage 0.2M sodium acetate