Praesto Pure45 is a plain agarose-based chromatography media designed for large scale biomolecule purification. Praesto Pure45 is the ideal base matrix for production of affinity chromatography resins with minimal unspecific interaction and long functional life time. The relatively small particle size (45µm) makes Praesto Pure45 the first choice for preparative high-resolution applications. Praesto Pure45 can also be directly used for purification by gel filtration of different large biomolecules such as viruses and plasmids.

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Principal Applications

  • Protein purification
  • Virus Application

Advantages

  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Not functionalized
Chemical stability All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents
Particle Size - µm 45 µm
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 200 cm/h
Exclusion limit for globular proteins 10 7 Daltons
pH stability, CIP (short term) 2 - 14
pH stability, working range 3 - 14
Recommended Storage 4 - 30 °C
Recommended Storage 20% ethanol