To support in the development and manufacture of biopharmaceuticals, Purolite has developed a range of pre-activated agarose resins. These resins enable manufacturers to couple their own ligands to develop affinity chromatography solutions. NHS pre-activated resins are available in three particle sizes - 45 μm, 65 μm and 90 μm.

Praesto NHS resins have been designed to offer a simple solution for the immobilization of ligands onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D proof of concept to larger scale bioprocess production columns. Praesto NHS resins couple ligands which have a primary amine. Praesto NHS incorporates a spacer chain which separates the ligand from the chromatography matrix enabling maximum accessibility to the ligand. The pre-activated NHS resin forms a stable amide linkage between the matrix and ligand resulting in very low ligand leakage and high caustic stability. A common application for NHS resins is immobilization of antibodies to create immunosorbents. Coupling is via primary amino groups. Many well documented references are publicly available.

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Principal Applications

  • Protein purification
  • Production of customized affinity chromatography media

Advantages

  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group NHS-ester
Chemical stability After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents
Particle Size - µm 90 µm
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 800 cm/h
Exclusion limit for globular proteins 10 7 Daltons
pH stability, CIP (short term) 2 - 13 (after coupling)
pH stability, working range 2 - 13 (after coupling)
Recommended Storage 100% IPA - 2 - 8 °C
Recommended Storage 20% EtoH (after coupling)