Highly cross linked pre-activated agarose base matrix
To support in the development and manufacture of biopharmaceuticals, Purolite has developed a range of pre-activated agarose resins. These resins enable manufacturers to couple their own ligands to develop affinity chromatography solutions. Epoxy pre-activated resins are available in three particle sizes - 45 μm, 65 μm and 90 μm.
Praesto Epoxy resins have been designed to offer a simple solution for the immobilization of ligands onto an agarose chromatography matrix, which can be utilized to make customized affinity media. This enables rapid scale-up from R&D proof of concept to larger scale bioprocess production columns. Praesto Epoxy resins offer the versatility to couple ligands through primary amine, hydroxyl and thiol groups. The Praesto Epoxy resin design incorporates a spacer which separates the ligand from the chromatography carrier enabling maximum efficiency of the ligand. The epoxide group forms a stable linkage between the matrix and ligand, which has very low ligand leakage and high caustic stability. Many well-documented references (published over several years) are publicly available.
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|Polymer Structure||Highly cross linked agarose|
|Chemical stability||After coupling - All commonly used aqueous buffers, 2M NaOH, 8M urea, 6M guanidine HCl, 30% isopropanol, 70% ethanol, 30% acetonitrile and commonly used detergents|
|Particle Size - µm||45 µm|
|Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar)||200 cm/h|
|Exclusion limit for globular proteins||10 7 Daltons|
|pH stability, CIP (short term)||2 - 13|
|pH stability, working range||2 - 13|
|Recommended Storage||100% IPA - 2 - 8 °C|
|Recommended Storage||20% EtoH (after coupling)|