Praesto Epoxy300  is an agarose based resin functionalised with epoxy groups. Epoxy groups form very stable covalent linkages with different protein surface groups as ε-NH2 in Lys or nucleophiles  (amino, thiol, phenolic). Immobilization is performed under very mild experimental conditions of pH and temperature.  Praesto Epoxy300 is particularly suitable for very large enzymes (>100KDA) and multimeric enzymes. Praesto Epoxy300 is a very hydrophilic resin and can be used in batch reactors and columns.

Principal Applications

  • Covalent immobilization of enzymes
  • Highly hydrophilic
  • Suitable for very large enzymes

Systems

  • Suitable for batch and column configuration

Typical Packaging

  • 10ml
  • 25ml
  • 100ml

Typical Physical & Chemical Characteristics:

Functional Group Epoxy
Supplied as Wet in 100% IPA
pH limit stability 5 - 9
Optimal storage condition 2 - 8 °C
Expiry date (from date of manufacture) 6 months
Volume capacity (min.) 10 ummol/ml of settled resin
Particle size (90% in Range) 200 - 400 µm
Polymer Structure Agarose