Lifetech ECR8409F is a methacrylate polymer functionalised with amino groups on a long spacer (C6). It is used for covalent enzyme immobilization by pre-activation of the resin with glutaraldehyde and to subsequently form very stable covalent linkages with different protein groups (amino, thiol, phenolic) under very mild experimental conditions of pH and temperature, at low ionic buffer strength. It can also be used for  enzyme immobilization by ionic interaction of the ionizable surface aminoacids (Lys, Arg, His, Asp, Glu) with the charged amines on the polymer. Lifetech ECR8409F main features are the medium porosity, the hydrophilicity and its optimal use in batch reactors.

Principal Applications

  • Covalent immobilization of enzymes (requires resin pre-activation with glutaraldehyde)
  • Can be used also for direct ionic enzyme immobilization
  • Ionic immobilization requires pH adjustment (resin is supplied at neutral pH)

Advantages

  • Medium porosity
  • Easy covalent enzyme immobilization
  • Adjustable pH for ionic immobilization

Systems

  • Particularly suitable for batch configuration
  • Optimal for all applications in aqueous media

Regulatory Approvals

  • ResAP(2004)3 Status (food grade)
  • Halal
  • Kosher
  • TSE/BSE/GMO free

Typical Packaging

  • 50g
  • 250g
  • 1Kg

Typical Physical & Chemical Characteristics:

Appearance White to off white beads
Functional Group Amino (long spacer)
Supplied as Wet
Particle Size Range 150 - 300 µm
Pore Diameter 600 - 1200 Å
Total moisture 62 - 72 %
pH limit stability 3 - 10
Recommended Storage 2 - 20 °C
Expiry date (from date of manufacture) 5 years