Lifetech ECR8215F is a methacrylate polymer functionalised with epoxy groups, used for covalent enzyme immobilization. Epoxy groups form very stable covalent linkages with different protein surface groups as ε-NH2 in Lys or nucleophiles  (amino, thiol, phenolic). Immobilization is performed under very mild experimental conditions of pH and temperature, at high ionic buffer strength.  Lifetech ECR8215F main features are the very high porosity, the hydrophilicity and it is optimal for use in batch reactors. 

Principal Applications

  • Covalent immobilization of enzymes
  • Epoxides form very stable covalent linkages
  • Suitable for immobilization of a wide range of enzymes (including hydrolases)
  • Coupling of specific ligands to create affinity resin

Advantages

  • High porosity
  • Easy covalent multipoint immobilization
  • Hydrophilic resin
  • Optimal for use in continuous column reactors

Systems

  • Particularly suitable for batch configuration
  • Optimal for all applications in aqueous media

Regulatory Approvals

  • ResAP(2004)3 Status (food grade)
  • Halal
  • Kosher
  • TSE/BSE/GMO free

Typical Packaging

  • 50g
  • 250g
  • 5Kg

Typical Physical & Chemical Characteristics:

Appearance White to off white beads
Functional Group Epoxy
Supplied as Wet
Particle Size Range 150 - 300 µm
Pore Diameter 1200 - 1800 Å
Total moisture 70 - 80 %
pH limit stability 5 - 9
Recommended Storage 2 - 8 °C
Expiry date (from date of manufacture) 6 months