AP HT Column (5ml)
Highly cross linked agarose Protein A affinity resin
Praesto HT columns are designed for efficient resin screening, as well as for optimization and verification. HT Columns from Purolite Life Sciences streamline your separations, and are available pre-packed with any Praesto Affinity or Ion Exchange Chromatography agarose resin.
Columns are available in 1 ml and 5 ml bed volumes with a 2.5 cm bed height. HT Columns are compatible with all commonly-used chromatography systems, due to universal 1/16" connectors. HT Columns are constructed of polypropylene, preventing interaction with biological molecules and ensuring biocompatibility.
Praesto AP is a Protein-A resin designed on a novel high flow agarose backbone which uses a recombinant alkaline-tolerant Protein-A ligand expressed in E.coli. The Protein-A ligand used for Praesto AP resulted from protein engineering of one of the IgG-binding domains of Protein-A. In particular, the amino acids sensitive to alkali conditions were identified and then substituted with more stable ones to deliver superior alkaline stability which provides hundreds of re-cycles. Praesto AP addresses today s late stage clinical manufacturing challenges for high productivity together with cost-efficient MAb capture. It is purpose-designed and evaluated for the production of late phase clinical trial material, PIII and commercial manufacture, where typically hundreds of reuse cycles are performed.
CONTACT A SPECIALIST
|Polymer Structure||Highly cross linked agarose|
|Functional Group||Protein A|
|Packed bed height||25 mm|
|Column volume||5 ml|
|Connector||Universal 10.32 (1/16") UNF Threads|
|Chemical stability||All commonly used aqueous buffers, pH 1-14, organic solvents|
|Avoid||Halogenated organic solvents, hexane|
|Working pressure (max.)||5 bar|
|Particle Size - µm||85 µm|
|Dynamic Binding Capacity (min.)- 3 minutes residence time||25 hlgG/ml|
|pH stability, CIP (short term)||2 - 12|
|pH stability, working range||3 - 10|
|Recommended Storage||2 - 8 °C|
|Recommended Storage||20% ethanol|