Macroporous Strong Base Anion
Chromalite A860/5952 is ideal for isolation of proteins, especially for proteins from milk and diary products. The charge on the protein is dependent of the working pH, resulting in three situations:
pH = IEP (iso-electric-point) = Protein is neutral
pH <IEP = Protein is positively charged
pH >IEP = Protein is negatively charged
By selecting the right pH conditions, selective adsorption of a protein can be achieved. Optimal protein separation is achieved when the target protein possesses a charge that is opposite to the other proteins in the solution. Under these conditions, protein will selectively adsorb on an IEX resin. A well-known example for this approach is the separation of Lactoferrin from whey using a cation exchanger. A similar approach can be applied to other proteins. Conditions are adjusted in such a way that the target protein possesses a negative charge, while the untargeted proteins are positively charged.
Chromalite A860/5952 is an ideal choice, due to its hydrophilic, protein-compatible matrix. The resin rigidity and particle size also allows packing in industrial columns.
|Functional Group||Quaternary Ammonium|
|Supplied as||Wet in Cl- form|
|Volume capacity (min.)||0.8 eq/l|
|Weight capacity (min.)||4 eq/Kg|
|Particle size (90% in Range)||425 - 1200 µm|
|Total moisture||66 - 72 %|
|pH limit stability||1 - 14|
|Optimal storage condition||2 - 20 °C|
|Expiry date (from date of manufacture)||5 years|